The Effects of Platelet-Rich-Plasma Gel Application to the colonic anastomosis in Hyperthermic intraperitoneal chemotherapy: An Experimental Rat Model

  General Surgery

  SÖNMEZ OCAK, Ömer Faruk Bük, Buğra Genç, Bahattin Avcı, Hatice Olger Uzuner, Salih Burak Gündoğdu

Now published in International Wound Journal doi: 10.1111/iwj.13207

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Abstract

Background and aims: Hyperthermic intraperitoneal chemotherapy (HIPEC) has cytotoxic effects on tumor cells but also negative impacts on anastomotic healing. Platelet-Rich-Plasma (PRP) is used for wound care but data about effects on gastrointestinal anastomosis is limited. In this experimental study we aimed to investigate the effects of PRP application on colon anastomosis in rats those performed HIPEC with cisplastin. Matherial and methods: Five rats were sacrified to obtain PRP gel.Thirty rats were divided into three groups; Group 1:Control Group, Group 2 : Colon anastomosis and HIPEC with Cisplatin and Group 3: Colon anastomosis enhanced by PRP and HIPEC with Cisplatin. The rats were re-operated on postoperative day seven and anastomotic bursting pressure (ABP) were recorded. Also tissue samples were taken for Hydroxyproline assesment and histopathological examination Results: There were significant difference in ABP between Group 2 and Group 3,and also those groups had lower ABP compared to control group. Group 3 had significantly higher hydroxyproline levels and had better histopathological findings than group 2. Conclusion: According to our findings, we suggest that PRP application improves the anastomotic healing by increasing anastomotic bursting pressure,hydroxyproline levels and decreasing inflammatory response. Further clinical studies are needed to prove our hypothesis

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