The distribution of hepatitis C virus genotypes in patients with hepatocellular carcinoma

  Gastroenterology & Hepatology Infectious Diseases Microbiology (medical)

  Ayfer Bakır, Nuran Karabulut, Sema Alaçam, Barış Bakır, Ali Ağaçfidan

Now published in Asian Journal of Medical Sciences doi: 10.3126/ajms.v10i6.25720

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Abstract

Objective Hepatic cirrhosis develops within 20-30 years in approximately 20% of individuals who are chronically infected with hepatitis C virus (HCV). The rate of hepatocellular carcinoma (HCC) development in cirrhotic patients is 1-7% per year. This study aimed to determine the distribution of HCV genotypes in patients with HCV-associated HCC in our region and thus to contribute to the epidemiology of HCV. Materials and Methods HCC patients who were referred to the virology laboratory for HCV genotype identification between January 2013 and April 2018 were included in this study. HCV RNA extraction was performed from plasma samples using the Qiagen kit (Germany). The products obtained after extraction was amplified using biotin-labeled primers selected from the 5'UTR non-coding region of HCV using the AMPLYQUALITY HCV-PM Bio kit (AB ANALITICA, Italy). Genotyping of HCV was performed by a commercial reverse hybridization line probe-based assay (AMPLIQUALITY HCV-TS, AB Analitica, Italy). Results Seventeen patients with HCC diagnosed who were analyzed HCV genotypes were included in the study. The median age of patients with HCC, consisting of nine (53%) male and eight (47%) female patients, was 67 years. The median HCV RNA viral load level of the patients was 731112 IU/ml. Genotype 1 was detected in all 17 patients with HCC. When evaluating subtypes of genotype 1, Genotype 1b was detected in 16 (94%) of the patients. The HCV subtyping in one patient (6%) could not be performed. Conclusion As a result, genotype 1b, one of the major risk factors for HCC, was detected in 94% of the patients included in the study. This study, in accordance with the literature, shows a high association between the development of HCC and genotype 1b in patients with chronic HCV.

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